|
Common name |
B-Tg(mArtn) MC38 | Catalog number |
322092 |
| Aliases | neublastin | Disease | Colon carcinoma |
|
Organism |
Mouse |
Strain | C57BL/6 |
| Tissue types | Colon | Tissue | Colon |
Description
The exogenous promoter and mouse Artn coding sequence were inserted into the mouse genome randomly. Mouse Artn can be secreted by B-Tg(mArtn) MC38 cells.
B-Tg(mArtn) MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.
Gene targeting strategy for B-Tg(mArtn) MC38 cells. The exogenous promoter and mouse Artn coding sequence were inserted into the mouse genome randomly.
Protein expression analysis
Artn expression analysis in B-Tg(mArtn) MC38 cells by ELISA. Mouse Artn was detected in the supernatant of B-Tg(mArtn) MC38 cells but not wild-type MC38 cells. The 2-E09 clone of B-Tg(mArtn) MC38 cells was used for in vivo experiments
Subcutaneous homograft tumor growth of B-Tg(mArtn) MC38 cells. B-Tg(mArtn) MC38 cells (5x105) and wild-type MC38 cells (5x105) were subcutaneously implanted into C57BL/6N mice (female, 7-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-Tg(mArtn) MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.
mArtn expression analysis in tumor
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