Strain specific APOC3 expression analysis in homozygous B-hAPOC3 mice by ELISA. Serum were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hAPOC3 mice (H/H) and analyzed by ELISA with species-specific APOC3 ELISA kit. Human APOC3 was exclusively detectable in homozygous B-hAPOC3 mice but not in wild-type mice .

Strain specific analysis of APOC3 mRNA expression in wild-type C57BL/6 mice and B-hAPOC3 mice by RT-PCR. Liver RNA were isolated from wild type C57BL/6 mice (+/+) and homozygous B-hAPOC3 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human APOC3 primers. Mouse Apoc3 mRNA was detectable only in wild-type C57BL/6 mice. Human APOC3 mRNA was detectable only in homozygous B-hAPOC3 mice but not in wild-type mice.