Strain Name
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C57BL/6N-Apoc3tm1(APOC3)/Bcgen
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Common Name
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B-hAPOC3 mice
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Background
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C57BL/6N
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Catalog number
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112411
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Aliases
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APOC3: Apo-C3, ApoC-3, APOCIII
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Protein expression analysis
Strain specific APOC3 expression analysis in homozygous B-hAPOC3 mice by ELISA. Serum were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hAPOC3 mice (H/H) and analyzed by ELISA with species-specific APOC3 ELISA kit. Human APOC3 was exclusively detectable in homozygous B-hAPOC3 mice but not in wild-type mice .
mRNA expression analysis
Strain specific analysis of APOC3 mRNA expression in wild-type C57BL/6 mice and B-hAPOC3 mice by RT-PCR. Liver RNA were isolated from wild type C57BL/6 mice (+/+) and homozygous B-hAPOC3 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human APOC3 primers. Mouse Apoc3 mRNA was detectable only in wild-type C57BL/6 mice. Human APOC3 mRNA was detectable only in homozygous B-hAPOC3 mice but not in wild-type mice.