|Strain Name||C57BL/6-Il11tm1(IL11)/Bcgen||Common Name||B-hIL11 mice|
interleukin 11, AGIF, IL-11
NCBI Gene ID
Gene targeting strategy for B-hIL11 mice.
The exons 1~5 of mouse Il11 gene that encode the the full-length protein were replaced by human IL11 exons 1~5 in B-hIL11 mice.
mRNA expression analysis
Strain specific analysis of IL11 gene expression in WT and hIL11 mice by RT-PCR.
Mouse Il11 mRNA was detectable in splenocytes of wild-type (+/+) and heterozygous B-hIL11 mice (H/+). Human IL11 mRNA was detectable only in heterozygous B-hIL11 mice but not in wild-type mice.
Protein expression analysis
Strain specific IL11 expression analysis in heterozygous B-hIL11 mice by ELISA.
Serum was collected from wild-type mice (+/+) and heterozygous B-hIL11 mice (H/+), and analyzed by ELISA with species-specific IL11 ELISA kit. Mouse IL11 was detectable in wild-type mice and heterozygous B-hIL11 mice. Human IL11 was exclusively detectable in heterozygous B-hIL11 mice but not in wild-type mice. ND: not detected.