|Strain Name||C57BL/6-Il1rl2tm1(IL1RL2)Il1raptm1(hIL1RAP)/Bcgen||Common Name||B-hIL36R/hIL1RACP mice|
IL1RL2, IL-1Rrp2, IL-36R, IL1R-rp2, IL1RRP2, interleukin 1 receptor like 2;
IL1RAP; IL1RAcP; IL1R3; C3orf13
mRNA expression analysis
Strain specific IL1RACP expression analysis in homozygous B-hIL36R/hIL1RACP mice by flow cytometry. Monocytes, macrophages and neutrophils were collected from wild type (WT) mice (+/+) and homozygous B-hIL36R/hIL1RACP mice (H/H;H/H), and analyzed by flow cytometry with species-specific anti-hIL1RACP antibody. Human IL1RACP was exclusively detectable in homozygous B-hIL36R/hIL1RACP mice (H/H;H/H) but not in WT mice (+/+).
Blood routine test in B-hIL36R/hIL1RACP mice
Blood biochemistry tests of B-hIL36R/hIL1RACP mice. Serum from the C57BL/6 and B-hIL36R/hIL1RACP mice (n=8, 8 week-old) was collected and analyzed for levels of ALT and AST. There was no differences on either measurement between C57BL/6 and B-hIL36R/hIL1RACP mice, indicating that introduction of IL36R and IL1RACP in place of its mouse counterpart do not change ALT and AST levels or health of liver. Values are expressed as mean ± SEM.
IL36g-induced skin inflammation in B-hIL36R/hIL1RACP mice