请输入关键字
请输入关键字
订购
*国家
中国
美国
中国香港
中国澳门
中国台湾
阿尔巴尼亚
阿尔及利亚
阿根廷
阿拉伯联合酋长国
阿鲁巴
阿曼
阿塞拜疆
阿森松岛
埃及
埃塞俄比亚
爱尔兰
爱沙尼亚
安道尔
安哥拉
安圭拉
安提瓜和巴布达
奥地利
奥兰群岛
澳大利亚
巴巴多斯
巴布亚新几内亚
巴哈马
巴基斯坦
巴拉圭
巴勒斯坦领土
巴林
巴拿马
巴西
白俄罗斯
百慕大
保加利亚
北马里亚纳群岛
贝宁
比利时
冰岛
波多黎各
波兰
波斯尼亚和黑塞哥维那
玻利维亚
伯利兹
博茨瓦纳
不丹
布基纳法索
布隆迪
朝鲜
赤道几内亚
丹麦
德国
迪戈加西亚岛
东帝汶
多哥
多米尼加共和国
多米尼克
俄罗斯
厄瓜多尔
厄立特里亚
法国
法罗群岛
法属波利尼西亚
法属圭亚那
法属南部领地
梵蒂冈
菲律宾
斐济
芬兰
佛得角
福克兰群岛
冈比亚
刚果(布)
刚果(金)
哥伦比亚
哥斯达黎加
格恩西岛
格林纳达
格陵兰
格鲁吉亚
古巴
瓜德罗普
关岛
圭亚那
哈萨克斯坦
海地
韩国
荷兰
荷属加勒比区
荷属圣马丁
黑山
洪都拉斯
基里巴斯
吉布提
吉尔吉斯斯坦
几内亚
几内亚比绍
加拿大
加纳
加纳利群岛
加蓬
柬埔寨
捷克
津巴布韦
喀麦隆
卡塔尔
开曼群岛
科科斯(基林)群岛
科摩罗
科索沃
科特迪瓦
科威特
克罗地亚
肯尼亚
库克群岛
库拉索
拉脱维亚
莱索托
老挝
黎巴嫩
立陶宛
利比里亚
利比亚
联合国
列支敦士登
留尼汪
卢森堡
卢旺达
罗马尼亚
马达加斯加
马恩岛
马尔代夫
马耳他
马拉维
马来西亚
马里
马其顿
马绍尔群岛
马提尼克
马约特
毛里求斯
毛里塔尼亚
美国本土外小岛屿
美属萨摩亚
美属维尔京群岛
蒙古
蒙特塞拉特
孟加拉国
秘鲁
密克罗尼西亚
缅甸
摩尔多瓦
摩洛哥
摩纳哥
莫桑比克
墨西哥
纳米比亚
南非
南极洲
南乔治亚和南桑威奇群岛
南苏丹
瑙鲁
尼加拉瓜
尼泊尔
尼日尔
尼日利亚
纽埃
挪威
诺福克岛
帕劳
皮特凯恩群岛
葡萄牙
日本
瑞典
瑞士
萨尔瓦多
萨摩亚
塞尔维亚
塞拉利昂
塞内加尔
塞浦路斯
塞舌尔
沙特阿拉伯
圣巴泰勒米
圣诞岛
圣多美和普林西比
圣赫勒拿
圣基茨和尼维斯
圣卢西亚
圣马丁岛
圣马力诺
圣皮埃尔和密克隆群岛
圣文森特和格林纳丁斯
斯里兰卡
斯洛伐克
斯洛文尼亚
斯瓦尔巴和扬马延
斯威士兰
苏丹
苏里南
所罗门群岛
索马里
塔吉克斯坦
泰国
坦桑尼亚
汤加
特克斯和凯科斯群岛
特里斯坦-达库尼亚群岛
特立尼达和多巴哥
突尼斯
图瓦卢
土耳其
土库曼斯坦
托克劳
瓦利斯和富图纳
瓦努阿图
危地马拉
委内瑞拉
文莱
乌干达
乌克兰
乌拉圭
乌兹别克斯坦
希腊
西班牙
西撒哈拉
新加坡
新喀里多尼亚
新西兰
匈牙利
休达及梅利利亚
叙利亚
牙买加
亚美尼亚
也门
伊拉克
伊朗
以色列
意大利
印度
印度尼西亚
英国
英属维尔京群岛
英属印度洋领地
约旦
越南
赞比亚
泽西岛
乍得
直布罗陀
智利
中非共和国
*省份
*城市
*姓名
*电话
*单位
*职位
*邮箱
*请输入验证码
*验证码
B-hTSLP/hTSLPR mice plus
Strain name Non-disclosure  Common Name  B-hTSLP/hTSLPR mice plus
Background C57BL/6 Catalog number 112744
Aliases  TSLP: NA;CRLF2: CRL2Y, TSLPR

Protein expression analysis-TSLP



from clipboard

Strain specific TSLP expression analysis in wild type C57BL/6 mice, homozygous B-hTSLP/hTSLPR mice and B-hTSLP/hTSLPR mice plus by ELISA. Calcipotriol (MC903) was dissolved in ethanol and topically applied on ears of wild type C57BL/6 mice, homozygous B-hTSLP/hTSLPR mice and B-hTSLP/hTSLPR mice plus for 7 days. Ear grinding supernatant from the three strain of mice were analyzed by ELISA. Mouse TSLP was only detectable in wild type C57BL/6 mice. Human TSLP was detectable in homozygous B-hTSLP/hTSLPR mice and B-hTSLP/hTSLPR mice plus but not in wild type mice.


Detection of longer and shorter isoform expression of human TSLP by RT-PCR


from clipboard

Longer and shorter isoform of human TSLP were detectable in B-hTSLP/hTSLPR mice plus by RT-PCR and sequencing. Ear tissues were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hTSLP/hTSLPR mice plus (H/H). A. Primers were designed to detect longer isoform (lfTSLP) and shorter isoform (sfTSLP) of human TSLP; B. LfTSLP mRNA was detectable in B-hTSLP/hTSLPR mice plus, but not in wild-type mice; C. SfTSLP mRNA was detectable in B-hTSLP/hTSLPR mice plus, but not in wild-type mice. The sequencing results of the shorter isoform PCR products confirmed that the sequences amplified in mice were consistent with those in the database.


Protein expression analysis of TSLPR in spleen

from clipboard

Data from Huabo Biopharm Co. Ltd.


Mouse and human TSLPR expression analysis in splenocytes. Splenocytes were collected from wild type C57BL/6 mice, homozygous B-hTSLP/hTSLPR mice and B-hTSLP/hTSLPR mice plus. TSLPR expressed on cDC, pDC and non DCs were analyzed by flow cytometry with species-specific anti-TSLPR antibody. Mouse TSLPR were detectable on cDC, pDC and non DCs of wild type C57BL/6 mice, but not on the cells of TSLPR humanized mice. Human TSLPR was detectable on cDC, pDC and non DCs of B-hTSLP/hTSLPR mice and B-hTSLP/hTSLPR mice plus, but not on the cells of wild type C57BL/6 mice.


Protein expression analysis of TSLPR in bone marrow

from clipboard

Data from Huabo Biopharm Co. Ltd.


Mouse and human TSLPR expression analysis in bone marrow. Bone marrow cells were collected from wild type C57BL/6 mice, homozygous B-hTSLP/hTSLPR mice and B-hTSLP/hTSLPR mice plus. TSLPR expressed on bone marrow cells were analyzed by flow cytometry with species-specific anti-TSLPR antibody. Mouse TSLPR were detectable on cDC, pDC and non DCs in wild type C57BL/6 mice, but not on the cells of TSLPR humanized mice. Human TSLPR was detectable on cDC, pDC and non DCs of B-hTSLP/hTSLPR mice and B-hTSLP/hTSLPR mice plus, but not on the cells of wild type C57BL/6 mice.


Protein expression analysis of hTSLPR in cDC1 from bone marrow


from clipboard

Data from Huabo Biopharm Co. Ltd.

Human TSLPR expression analysis in cDC1 from bone marrow. Bone marrow were collected from wild type C57BL/6 mice, homozygous B-hTSLP/hTSLPR mice and B-hTSLP/hTSLPR mice plus. Human TSLPR expressed on cDC1 were analyzed by flow cytometry with species-specific antibodies. Human TSLPR was highly expressed on the cDC1 in B-hTSLP/hTSLPR mice and B-hTSLP/hTSLPR mice plus. 


Analysis of leukocytes cell subpopulation in spleen


from clipboard


Analysis of spleen leukocyte subpopulations by flow cytometry. Splenocytes were isolated from female C57BL/6 and B-hTSLP/hTSLPR mice plus (n=3, 9-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, DCs, neutrophils, monocytes and macrophages in homozygous B-hTSLP/hTSLPR mice plus were similar to those in the C57BL/6 mice, demonstrating that TSLP,TSLPR and IL7R humanized does not change the overall development, differentiation or distribution of these cell types in splenocytes. Values are expressed as mean ± SEM.

Analysis of T cell subpopulation in spleen

from clipboard


Analysis of spleen T cell subpopulations by flow cytometry. Splenocytes were isolated from female C57BL/6 and B-hTSLP/hTSLPR mice plus (n=3, 9-week-old).  Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for CD3+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD4+ T cells, CD8+ T cells and Tregs in homozygous B-hTSLP/hTSLPR mice plus were similar to those in the C57BL/6 mice, demonstrating that TSLP,TSLPR and IL7R humanized does not change the overall development, differentiation or distribution of these T cell types in spleen. Values are expressed as mean ± SEM.

Analysis of leukocytes cell subpopulation in blood

from clipboard


Analysis of blood leukocyte subpopulations by flow cytometry. Blood were isolated from female C57BL/6 and B-hTSLP/hTSLPR mice plus (n=3, 9-week-old). Flow cytometry analysis of the blood was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, dendritic cells, neutrophils, monocytes and macrophages in homozygous B-hTSLP/hTSLPR mice plus were similar to those in the C57BL/6 mice, demonstrating that TSLP,TSLPR and IL7R humanized does not change the overall development, differentiation or distribution of these cell types in blood. Values are expressed as mean ± SEM.

Analysis of T cell subpopulation in blood

from clipboard


Analysis of blood T cell subpopulations by flow cytometry. Blood were isolated from female C57BL/6 and B-hTSLP/hTSLPR mice plus (n=3, 9-week-old).  Flow cytometry analysis of the blood was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for CD3+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD4+ T cells, CD8+ T cells and Tregs in homozygous B-hTSLP/hTSLPR mice plus were similar to those in the C57BL/6 mice, demonstrating that TSLP,TSLPR and IL7R humanized does not change the overall development, differentiation or distribution of these T cell types in blood. Values are expressed as mean ± SEM.

Analysis of leukocytes cell subpopulation in lymph node

from clipboard


Analysis of lymph node leukocyte subpopulations by flow cytometry. Lymph nodes were isolated from female C57BL/6 and B-hTSLP/hTSLPR mice plus (n=3, 9-week-old). Flow cytometry analysis of the lymph node was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, dendritic cells, neutrophils, monocytes and macrophages in homozygous B-hTSLP/hTSLPR mice plus were similar to those in the C57BL/6 mice, demonstrating that TSLP, TSLPR and IL7R humanized does not change the overall development, differentiation or distribution of these cell types in lymph node. Values are expressed as mean ± SEM.

Analysis of T cell subpopulation in lymph node

from clipboard


Analysis of lymph node T cell subpopulations by flow cytometry. Lymph nodes were isolated from female C57BL/6 and B-hTSLP/hTSLPR mice plus (n=3, 9-week-old).  Flow cytometry analysis of the lymph node was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for CD3+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD4+ T cells, CD8+ T cells and Tregs in homozygous B-hTSLP/hTSLPR mice plus were similar to those in the C57BL/6 mice, demonstrating that TSLP, TSLPR and IL7R humanized does not change the overall development, differentiation or distribution of these T cell types in lymph node. Values are expressed as mean ± SEM.

Analysis of leukocytes cell subpopulation in thymus

from clipboard


Analysis of thymus leukocyte subpopulations by flow cytometry. Thymuses were isolated from female C57BL/6 and B-hTSLP/hTSLPR mice plus (n=3, 9-week-old). Flow cytometry analysis of the thymus was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, dendritic cells, neutrophils, monocytes and macrophages in homozygous B-hTSLP/hTSLPR mice plus were similar to those in the C57BL/6 mice, demonstrating that TSLP, TSLPR and IL7R humanized does not change the overall development, differentiation or distribution of these cell types in thymus. Values are expressed as mean ± SEM.

Analysis of T cell subpopulation in thymus

from clipboard


Analysis of thymus T cell subpopulations by flow cytometry. Thymuses were isolated from female C57BL/6 and B-hTSLP/hTSLPR mice plus (n=3, 9-week-old).  Flow cytometry analysis of the thymus was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for CD3+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD4+ T cells, CD8+ T cells and Tregs in homozygous B-hTSLP/hTSLPR mice plus were similar to those in the C57BL/6 mice, demonstrating that TSLP, TSLPR and IL7R humanized does not change the overall development, differentiation or distribution of these T cell types in thymus. Values are expressed as mean ± SEM.


Functional analysis

from clipboard

Data from Huabo Biopharm Co. Ltd.


Mouse TARC was respectively induced with human TSLP and mouse TSLP in wild type C57BL/6 mice, homozygous B-hTSLP/hTSLPR mice and B-hTSLP/hTSLPR mice plus. Dendritic cells were respectively induced with FLT3L from bone marrow of the three strains and stimulated with human TSLP or mouse TSLP in vitro. Concentration of mouse TARC secreted from DCs was assayed by ELISA. Mouse TARC was successfully induced with human TSLP, but not mouse TSLP in homozygous B-hTSLP/hTSLPR mice and B-hTSLP/hTSLPR mice plus. The level of mTARC in B-hTSLP/hTSLPR mice plus was higher than that in B-hTSLP/hTSLPR mice plus.  Meanwhile mouse TARC was successfully induced with mouse TSLP, but not human TSLP in wild-type C57BL/6 mice. Results indicated that TSLP and TSLPR are not cross-reactive between mouse and human. Human TSLP can activate the dendritic cells of B-hTSLP/hTSLPR mice and B-hTSLP/hTSLPR mice plus. 


Growth curve 


from clipboard


Growth curve of B-hTSLP/hTSLPR mice plus. Eight weeks old of mice were grouped (10 males and 10 females, respectively). Body weight was measured on the same day of every week and lasted for 12 weeks. The lowest and highest values of mouse body weight in the table were calculated from the mean ± SD. The growth curve conforms to the normal distribution and the probability of random error falling within ± SD is 68%.

Hematology analysis

from clipboard

Complete blood count (CBC) of B-hTSLP/hTSLPR mice plus. Values are expressed as mean ± SD.


Biochemistry analysis

from clipboard

Blood biochemical test of B-hTSLP/hTSLPR mice plus. Values are expressed as mean ± SD.


Gross anatomy of female B-hTSLP/hTSLPR mice plus

from clipboard

The organs of female B-hTSLP/hTSLPR mice plus (12-week-old, n=10). 


Gross anatomy of male B-hTSLP/hTSLPR mice plus


from clipboard

The organs of male B-hTSLP/hTSLPR mice plus (12-week-old, n=10). 


Organ weight

from clipboard

Average weight of the main organs of B-hTSLP/hTSLPR mice plus. 


Histopathological analysis

from clipboard


In vivo efficacy of anti-human TSLP antibody in a new mouse asthma model


from clipboard

Analysis of inflammatory cells in BALF by FACS. Mouse asthma model was induced in B-hTSLP/hTSLPR mice and treated with anti-human TSLP antibody (tezepelumab, synthesized in house). BALF was collected at the end of the experiment to detect infiltrated inflammatory cells in lung tissue. The results showed that CD45+ cells, eosinophils and neutrophils in the group (G2) treated with anti-human TSLP antibody decreased significantly when compared with the group (G1) treated with isotype antibody. Values are expressed as mean ± SEM.


In vivo efficacy of anti-human TSLP antibody in a new mouse asthma model


from clipboard

OVA specific IgE in serum and TARC in BALF were  significantly reduced in the mouse asthma model treated with anti-TSLP antibody. Serum was collected at the study endpoint. IgE and TARC levels were analyzed by ELISA. The results showed that the levels of OVA specific IgE and TARC in mice  treated with tezepelumab (in house) was lower than that in untreated mice. Values are expressed as mean ± SEM. TARC: thymic and activating regulatory chemokine, also known as CCL17 (C-C motif chemokine ligand 17).


In vivo efficacy of anti-human TSLP antibody in a new mouse asthma model

from clipboard

H&E staining of asthma-like model in B-hTSLP/hTSLPR mice plus. Lung tissues were collected at the study endpoint and analyzed with H&E staining. The results showed that compared to the untreated group (G1), the group of mice treated with tezepelumab (in house) showed a significant reduction in inflammatory infiltration and mucus secretion in lung tissue, indicating that B-hTSLP/hTSLPR mice provide a powerful preclinical model for in vivo evaluation of anti-human TSLP antibodies. Black arrow: inflammatory cells; black triangle: eosinophils; asterisk: mucus. Values are expressed as mean ± SEM. 


Experimental schedule for Induction of AD-like skin lesions and in vivo efficacy of anti-human TSLP antibody  


from clipboard


Experimental schedule for Induction of atopic dermatitis (AD)-like skin lesions and in vivo efficacy of anti-human TSLP antibody in B-hTSLP/hTSLPR mice plus. OXA was applied to ear skin of mice on day 0, and then challenge to the same site of skin nine times from days 7 to 25. Anti-human TSLP antibody tezepelumab (in house) was administered by intraperitoneal injection (n = 6). OXA: oxazolone.


In vivo efficacy of anti-human TSLP antibody in OXA induced AD-like mouse model 


from clipboard

Efficacy of anti-human TSLP antibody in B-hTSLP/hTSLPR mice plus. Mice in each group were treated with anti-hTSLP antibody tezepelumab (in house). (A) Statistical analysis of ear thickness in each group. Epidermis of ear began to desquamate from day 18. So the ear thicknesses were decreased from day 18 as shown in figure. (B) Body weight changes during the treatment. (C) Total IgE levels in serum. Serum was collected on day 26 and total IgE levels were measured by ELISA. (n = 6). Values are expressed as mean ± SEM.


H&E staining of ear skin in AD-like mouse model of B-hTSLP/hTSLPR mice plus


from clipboard

Effects of anti-human TSLP antibody on ear skin of the AD mouse model.(A) Hematoxylin and eosin (H&E) staining. (B) Thickness of ear epidermal skin. (C) Score of eosinophils infiltrated in ear epidermal skin.(D) Total score of ear epidermal skin. Ear thickness and infiltration scores of eosinophils in ear skin of the groups treated with Tezepelumab (in house) were decreased significantly compared to that in the isotype control, demonstrating that the B-hTSLP/hTSLPR mice plus provide a powerful preclinical model for in vivo evaluation of anti-human TSLP antibodies. Infiltration score of eosinophils: 1=slight; 2=mild; 3=moderate; 4=severe. The content of the pathology total score evaluation includes the following aspects: epidermal hyperplasia in skin, erosion/crusting, hyperkeratosis and parakeratosis; inflammatory cell infiltration in dermis and subcutaneous. AD: Atopic dermatitis.