Analysis of spleen leukocytes cell subpopulations in B-hHER2 mice
Analysis of spleen leukocyte subpopulations by FACS.Splenocytes were isolated from female C57BL/6 and B-hHER2 mice (n=3, 9 week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for CD45 population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, dendritic cells, granulocytes, monocytes and macrophages in homozygous B-hHER2 mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hHER2 in place of its mouse counterpart does not change the overall development, differentiation or distribution of these cell types in spleen. Values are expressed as mean ± SEM.
Analysis of spleen T cell subpopulations in B-hHER2 mice
Analysis of spleen T cell subpopulations by FACS.Splenocytes were isolated from female C57BL/6 and B-hHER2 mice (n=3, 9 week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for CD3 T cell population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of CD8+ T cells, CD4+ T cells and Treg cells in homozygous B-hHER2 mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hHER2 in place of its mouse counterpart does not change the overall development, differentiation or distribution of these T cell sub types in spleen. Values are expressed as mean ± SEM.
Analysis of lymph node leukocytes cell subpopulations in B-hHER2 mice
Analysis of lymph node leukocyte subpopulations by FACS.Leukocytes were isolated from female C57BL/6 and B-hHER2 mice (n=3, 9 week-old). Flow cytometry analysis of the leukocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for CD45 population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells and NK cells in homozygous B-hHER2 mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hHER2 in place of its mouse counterpart does not change the overall development, differentiation or distribution of these cell types in lymph node. Values are expressed as mean ± SEM.
Analysis of lymph node T cell subpopulations in B-hHER2 mice
Analysis of lymph node T cell subpopulations by FACS.Leukocytes were isolated from female C57BL/6 and B-hHER2 mice (n=3, 9 week-old). Flow cytometry analysis of the leukocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for CD3 T cell population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of CD8+ T cells, CD4+ T cells and Treg cells in homozygous B-hHER2 mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hHER2 in place of its mouse counterpart does not change the overall development, differentiation or distribution of these T cell sub types in lymph node. Values are expressed as mean ± SEM.
Analysis of blood leukocytes cell subpopulations in B-hHER2 mice
Analysis of blood leukocyte subpopulations by FACS.Blood cells were isolated from female C57BL/6 and B-hHER2 mice (n=3, 9 week-old). Flow cytometry analysis of the blood leukocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for CD45 population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, dendritic cells, granulocytes, monocytes and macrophages in homozygous B-hHER2 mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hHER2 in place of its mouse counterpart does not change the overall development, differentiation or distribution of these cell types in blood. Values are expressed as mean ± SEM.
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