|Gene symbol and name||
|Strain of origin||
|Coat color||Black||Dietary information||
Growth and reproduction diet for experimental mice
Homozygous generally embryonic lethal, behave abnormal blood vessel development. In this strain, iCre-ERT2 recombinase expression is under the control of Cdh5 promoter. An iCre-ERT2 sequence cassette was placed between the coding sequence of exon 2 and 5’UTR of the Cdh5 gene, and the expression of endogenous Cdh5 gene in mice was disrupted. When crossed with a strain containing a loxP-site flanked sequence of interest, and induced with Tamoxifen, Cre-mediated recombination will result in deletion of the floxed sequence in the offspring.
•Function research of genes
•Research on cardiovascular system, tumor and cell differentiation mechanism
Immunoﬂuorescent images of tdTomato and CD31 in the main organs of double-positive mice. CD31 is endothelial marker. Scale bar, 25 µm.
【1】 Payne S, De Val S, Neal A. Endothelial-Specific Cre Mouse Models. Arterioscler Thromb Vasc Biol. 2018 Nov;38(11):2550-2561. [PubMed: 30354251]
【2】Kogata N, Arai Y, Pearson JT, Hashimoto K, Hidaka K, Koyama T, Somekawa S, Nakaoka Y, Ogawa M, Adams RH, Okada M, Mochizuki N. Cardiac ischemia activates vascular endothelial cadherin promoter in both preexisting vascular cells and bone marrow cells involved in neovascularization. Circ Res. 2006 Apr 14;98(7):897-904. [PubMed: 16543497]
【3】Oberlin E, El Hafny B, Petit-Cocault L, Souyri M. Definitive human and mouse hematopoiesis originates from the embryonic endothelium: a new class of HSCs based on VE-cadherin expression. Int J Dev Biol. 2010;54(6-7):1165-73. [PubMed: 20711993]
【4】 Chen MJ, Yokomizo T, Zeigler BM, Dzierzak E, Speck NA. Runx1 is required for the endothelial to haematopoietic cell transition but not thereafter. Nature. 2009 Feb 12;457(7231):887-91. [PubMed: 19129762]
【5】Alva JA, Zovein AC, Monvoisin A, Murphy T, Salazar A, Harvey NL, Carmeliet P, Iruela-Arispe ML. VE-Cadherin-Cre-recombinase transgenic mouse: a tool for lineage analysis and gene deletion in endothelial cells. Dev Dyn. 2006 Mar;235(3):759-67. [PubMed: 16450386]