| Strain Name |
C57BL/6-Sp7tm1(icre)/Bcgen |
Stock No. | 110131 |
| Common name |
B-Sp7-F2A-iCre mice |
Gene symbol and name | Sp7(transcription factor 7), Osx |
| Strain of origin |
C57BL/6 |
Chromosome | 15 |
| Coat color | Black | Dietary information | Growth and reproduction diet for experimental mice |
|
NCBI Gene ID |
170574 | ||
Application
- Function research of genes
- This Sp7iCre model is an efficient tool to study various gene functions when crossed with mice with different loxP site-flanked genes of interest, especially in studies of bone development, osteoblast lineage, and Hedgehog/Wnt signaling.
Expression
Expressed gene
iCre, improved cre recombinase, bacteriophage P1
Site of expression
osteoblast lineage, embryos perichondrium, bone trabeculae, bone collars
Gene editing strategy
An F2A-iCre sequence cassette was placed between the coding sequence of exon 2 and 3’UTR of the Sp7 gene in C57BL/6 ES cells. This strain was maintained on a C57BL/6 genetic background.
Phenotype Analysis
IFT140 was deleted in mesenchyme by crossing with Osx-Cre mice. It
was found that Osx-Cre was active in odontoblasts, and the expression of IFT140
was specifically downregulated in the odontoblasts of Ift140 cKO mice in the
furcation area (Fig. 2)
Fig 2. IFT140 was deleted in odontoblasts in Ift140 cKO mice. D,
dentin; OD, odontoblasts; PDL, periodontal ligament. Scale bars: 100 µm.
(source: Rodda SJ et al.,2006)
Reference
1. Rodda SJ; McMahon AP. 2006. Distinct roles
for Hedgehog and canonical Wnt signaling in specification, differentiation and
maintenance of osteoblast progenitors. Development 133(16):3231-44. [PubMed:
16854976]
2. Canalis E; Parker K; Feng JQ; Zanotti S.
2013. Osteoblast lineage-specific effects of notch activation in the skeleton.
Endocrinology 154(2):623-34. [PubMed: 23275471]
[MGI Ref ID J:194661] Canalis E; Parker K; Feng JQ; Zanotti S. 2013.
Osteoblast lineage-specific effects of notch activation in the skeleton.
Endocrinology 154(2):623-34. [PubMed: 23275471]
3. Chen J; Shi Y; Regan J; Karuppaiah K; Ornitz
DM; Long F. 2014. Osx-Cre targets multiple cell types besides osteoblast
lineage in postnatal mice. PLoS One 9(1):e85161. [PubMed: 24454809]
4. Liu Y; Strecker S; Wang L; Kronenberg MS;
Wang W; Rowe DW; Maye P. 2013. Osterix-cre labeled progenitor cells contribute
to the formation and maintenance of the bone marrow stroma. PLoS One
8(8):e71318. [PubMed: 23951132]
5. Shekaran A; Shoemaker JT; Kavanaugh TE; Lin
AS; LaPlaca MC; Fan Y; Guldberg RE; Garcia AJ. 2014. The effect of conditional
inactivation of beta 1 integrins using twist 2 Cre, Osterix Cre and osteocalcin
Cre lines on skeletal phenotype. Bone 68:131-41. [PubMed: 25183373]
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