|Gene symbol and name||Tcf21 (transcription factor 21), Po, ep|
|Strain of origin||
|Coat color||Black||Dietary information||Growth and reproduction diet for experimental mice|
NCBI Gene ID
Cre, cre recombinase, bacteriophage P1
Site of expression
Bladder adult, Lung adult, mouse embryos in mesenchymal cells
Gene editing strategy
A iCreERT2-WPRE-pA sequence cassette was inserted downstream 5’UTR of the Tcf21 gene. This strain was maintained on a C57BL/6 genetic background.
Figure 2. Blocking SEV secretion from ECs delays AML progression. (A) The experimental design. (B–F) Survival curves of AML recipients with different genotypes. Cdh5-Cre;Vps33bfl/fl (B), Lepr-Cre;Vps33bfl/fl (C), Osx-CreER;Vps33bfl/fl (D), Pf4-Cre;Vps33bfl/fl (E), and Tcf21-CreER;Vps33bfl/fl (F) are shown (n = 5–8; **P < 0.01, ***P < 0.001, log-rank test). Experiments were conducted 3 times for validation.
They used 7 cell type–specific mouse Cre lines to conditionally knock out Vps33b in Cdh5+ or Tie2+ endothelial cells (ECs), Lepr+ BM perivascular cells, Osx+ osteoprogenitor cells, Pf4+ megakaryocytes, and Tcf21+ spleen stromal cells.
(source: Huang D et al.,2021)
1. Huang D, Sun G, Hao X, He X, Zheng Z, Chen C, Yu Z, Xie L, Ma S, Liu L, Zhou BO, Cheng H, Zheng J, Cheng T. ANGPTL2-containing small extracellular vesicles from vascular endothelial cells accelerate leukemia progression. J Clin Invest. 2021 Jan 4;131(1):e138986. [PubMed: 33108353]2. Akama T, Chun TH. Transcription factor 21 (TCF21) promotes proinflammatory interleukin 6 expression and extracellular matrix remodeling in visceral adipose stem cells. J Biol Chem. 2018 Apr 27;293(17):6603-6610. [PubMed: 29540474]