B-Tgfb1 cKO mice
|Gene symbol and name||Tgfb1(transforming growth factor, beta 1), Tgfb|
|Strain of origin||
|Coat color||Black||Dietary information||Growth and reproduction diet for experimental mice|
NCBI Gene ID
Gene editing strategy
A designed targeting vector containing 5’-homologous arm, loxP-Exon2-frt-Neo-frt-loxP, 3’-homologous arm, and DTA selection marker was introduced into C57BL/6 ES cells, replacing corresponding Tgfb1 exon 2 locus. The resulting chimeric animals were crossed to C57BL/6 mice to establish the colony.
TGFb1 produced by myeloid cells is required for GEMsmediated immunomodulation. Tgfb1f/fLysM-Cre and Tgfb1+/+LysMCre mice were gavaged with GEMs before the subsequent experiment.a Flow cytometry analysis of Foxp3 expression in CD4+ T cells in the MLN, IEL, and LPL. Bar graph shows data from three experiments combined. b RNA was extracted from the small intestines of indicated mice, and the expression level of IL-10 was assayed by qRT-PCR. c CD11c+ DCs were purified from the MLNs of GEM-treated Tgfb1f/fLysM-Cre and Tgfb1+/+LysM-Cre mice and incubated with naïve CD4+ T cells in Treg-polarizing condition. Numbers indicate the percentages of CD4+Foxp3+ T cells after 4 days co-culture. Bar graph shows data from three experiments combined. d–f Tgfb1f/fLysM-Cre and Tgfb1+/+LysMCre mice were gavaged with GEMs before DSS administration. d Body weight. e Colons length. f Representative images of H&E staining. g Tgfb1f/fLysM-Cre and Tgfb1+/+LysM-Cre mice were treated with GEMs before H. felis administration. Colonization of H. felis in the gastric tissue was measured by qRT-PCR.
(source: Zeng Z et al.,2019)
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3. Engle SJ; Ormsby I; Pawlowski S; Boivin GP; Croft J; Balish E; Doetschman T. 2002. Elimination of Colon Cancer in Germ-free Transforming Growth Factor Beta 1-deficient Mice. Cancer Res 62(22):6362-6. [PMID: 12438215]4. Zeng Z, Liu W, Luo S, Hu C, Xu N, Huang A, Xi T, Xing Y. Shape of gastrointestinal immunity with non-genetically modified Lactococcus lactis particles requires commensal bacteria and myeloid cells-derived TGF-β1. Appl Microbiol Biotechnol. 2019 May;103(9):3847-3861. [PMID: 30852661]