Gene targeting strategy
Gene targeting strategy for B-luc B16-F10 . The luciferase cDNA sequence with CAG promoter was inserted in the ROSA26 exon1 of wild-type B16-F10.
In vitro luciferase activity of B-luc B16-F10 cells
Subcutaneous homograft tumor growth of B-luc B16-F10 cells. B-luc B16-F10 cells and wild-type B16-F10 cells were subcutaneously implanted into C57BL/6 mice (female, 7-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B) Body weight. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-luc B16-F10 cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.